Fluorescence multicolor hologram recorded by using a macrolens array.
نویسندگان
چکیده
We present a new efficient method for obtaining multicolor digital Fresnel holograms of three-dimensional (3-D) objects emitting incoherent fluorescent light. For each emitted fluorescent light wavelength, a single monochromatic digital modified Fresnel hologram of the 3-D scene is generated. Using a macrolens array, only nine projections of the 3-D scene are captured in a single camera shot and are then digitally processed to yield the hologram. The 3-D reconstructed images from all the monochromatic holograms are composed into a single multicolor image of the 3-D scene. The proposed holographic technique is demonstrated by experiments.
منابع مشابه
Fluorescence incoherent color holography.
We present a new imaging method to record multicolor digital holograms from objects emitting fluorescent light. The fluorescent light specific to the emission wavelength of various fluorescent dyes after excitation of three dimensional (3D) objects is recorded on a digital monochrome camera after reflection from a diffractive optical element (DOE). For each wavelength of fluorescent emission, t...
متن کاملReview of three-dimensional holographic imaging by multiple-viewpoint-projection based methods.
Methods of generating multiple viewpoint projection holograms of three-dimensional (3-D) realistic objects illuminated by incoherent white light are reviewed in this paper. Using these methods, it is possible to obtain holograms with a simple digital camera, operating in regular light conditions. Thus, most disadvantages characterizing conventional digital holography, namely the need for a powe...
متن کاملSpecial multicolor illumination and numerical tilt correction in volumetric digital holographic microscopy.
We introduce a color imaging method in our digital holographic microscope system (DHM). This DHM can create color images of freely floating, or moving objects inside a large volume by simultaneously capturing three holograms using three different illumination wavelengths. In this DHM a new light source assembly is applied, where we use single mode fibers according to the corresponding wavelengt...
متن کاملMulticolor 3D meta-holography by broadband plasmonic modulation
As nanofabrication technology progresses, the emerging metasurface has offered unique opportunities for holography, such as an increased data capacity and the realization of polarization-sensitive functionality. Multicolor three-dimensional (3D) meta-hologram imaging is one of the most pursued applications for meta-hologram not yet realized. How to reduce the cross-talk among different colors i...
متن کاملIncoherent Digital Holographic Microscopy with Coherent and Incoherent Light
Holography is an attractive imaging technique as it offers the ability to view a complete three-dimensional volume from one image. However, holography is not widely applied to the regime of fluorescence microscopy, because fluorescent light is incoherent and creating holograms requires a coherent interferometer system. We review two methods of generating digital Fresnel holograms of threedimens...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Optics letters
دوره 33 13 شماره
صفحات -
تاریخ انتشار 2008